ABSTRACT
Objective@#To investigate the antigenic epitope prediction method of hemagglutinin (HA) protein of influenza A (H1N1) virus.@*Methods@#BALB/c mice were conventionally immunized with influenza H1N1 vaccine. The splenocytes from the immunized mouse were fused with SP2/0 mouse myeloma cell line, and then the antigen-specific monoclonal antibodies (mAbs) were obtained by screening hybridoma supernatants. ELISA blocking test was used to detect the blocking result of each monoclonal antibody, which was labeled by horseradish peroxidase (HRP). The light and heavy chain variable region genes of each antibody were cloned, the amino acid sites of the antibody-binding HA antigen epitope were predicted by computer simulation.@*Results@#Three hybridoma cell lines of stable secreting anti-H1N1 influenza virus HA protein were obtained.Three mAbs were divided into two categories by ELISA blocking tests, which were divided into two categories according to preliminary results of computer simulation.@*Conclusions@#ELISA blocking test and computer simulation prediction can prove each other in predication of the antigenic epitopes of HA protein of H1N1 influenza virus.
ABSTRACT
Aim To investigate the effects of red yeast rice capsules containing coenzyme Q10 on femur with an animal model of osteoporosis, which was induced by OVX with D-galactose in rats, and the results were compared with those obtained from diethylstilbestrol. Methods Three-month old female Sprague-Dawley rats were randomly divided into sham group ( CON ) , ovariectomized group ( OVX ) , model group ( MOD ) , diethylstilbestrol group( DES) , and red yeast rice cap-sule group( RYR) . After 60 days, the left femurs were collected for Ca, P and hyp measurement, while the right femurs were performed with three-point bending test and micro-CT evaluation, respectively. Results Compared with CON group, MOD group had a signifi-cant increase in body weight, Tb. Sp, SMI and signifi-cant decrease in maximum load, stiffness, maximum strength, break strength, elastic modulus, Ca, P, Hyp contents and indicators of BV/TV, Tb. N, BMD, Conn-Dens. On the other hand, compared with MOD group, RYR group had a lower body weight and all bone biomechanics indexes were increased without sta-tistically significant difference. At the same time, the content of Ca, P and indicators of BV/TV, Tb. N, Tb. Th, BMD, Conn-Dens increased significantly;yet Tb. Sp decreased significantly. In DES group, the results of indicators were consistent with those for RYR group. In addition, compared with DES group, in RYR group body weight decreased significantly;the content of Ca, P and indicators of BV/TV, Tb. N, Tb. Th, Conn-Dens were significantly higher, and Tb. Sp, SMI were significantly lower. Conclusions Significant bone loss and deteriorated mechanical properties of femur can be observed in animal model of osteoporosis induced by OVX combined with D-galactose. Red yeast rice cap-sules containing coenzyme Q10 show effective prevention effects. Furthermore, red yeast rice capsules(0. 5 tab-let·kg-1 ) have better effect on increasing the number of trabecular bone than diethylstilbestrol ( 30 μg · kg-1 ) does.
ABSTRACT
Objective: To investigate the effects of Pingzhi Jianxin tea(平脂健心茶) on treating hyperl ipaemia and its mechanisms.Methods:One hundred and twenty cases with hyperlipaemia were randomly divided into trea tme nt group and control group, the treatment group was treated with Pingzhi Jianxin tea and the control group was t reated with gingko nut leaf tablet.The curative effects between two groups were compared.Meanwhile, experimental study on animal model of hyperlipaemia was conducted.Results:The total effective rates in depressing blood lipids and viscosity in treatment group was 92.5% and in control group was 72.5%.The total effective rates in electrocardiogram improvements were 65.5% and 56.3% respectively.The difference between two groups was significant(P<0.01 and P<0.05)． In experimental study the blood lipid of hyperlipaemia rats was reduced after taking Pingzhi Jianxin tea and this action in treatment group was superior to that in control group (P<0.01). Conclusions:The curative effects of Pingzhi Jianxin tea on treating hyperlipaemia and improving myocardial ischemia are confirmed and if used for a long time there is not harmful effect.